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addgene pt3 ef1a yaps127a  (Addgene inc)


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    Structured Review

    Addgene inc addgene pt3 ef1a yaps127a
    Addgene Pt3 Ef1a Yaps127a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pt3+ef1a+yaps127a/pT-3EF1a-Yap+S127A+(Plasmid+%2346049)/pm40578361-599-118-118
    Average 93 stars, based on 12 article reviews
    addgene pt3 ef1a yaps127a - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: An Inflammatory Checkpoint Generated by <i>IL1RN</i> Splicing Offers Therapeutic Opportunity for <i>KRAS</i>-Mutant Intrahepatic Cholangiocarcinoma
    Article Snippet: OCTOBER 2023 CANCER DISCOVERY | 2249

    Article Title: A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma
    Article Snippet: The plasmids encoding LDLR were gifts from Marvin Bentley Lab (Addgene plasmid # 162717). .. For transient transfection, plasmids were transiently transfected into ICC cells using Lipofectamine 3000 reagents according to the manufacturer’s instructions. pT3-myr-AKT-HA (Addgene plasmid # 31789) and pT3-EF1a-YapS127A (Addgene plasmid # 46049) were gifts from Dr. Xin Chen. .. Lentivirus containing FGFR2-AHCYL1 fusion plasmid and the corresponding vector GV492 were constructed by Genechem Co., Ltd. ICC cell lines, RBE, HuCCT1, and HCCC-9810 were transfected with the FGFR2-AHCYL1 lentivirus (multiplicity of infection (MOI)) according to the manufacturer’s instructions.

    Article Title: A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma.
    Article Snippet: The plasmids encoding LDLR were gifts fromMarvin Bentley Lab (Addgene plasmid # 162717). .. For transient transfection, plasmids were transiently transfected into ICC cells using Lipofectamine 3000 reagents according to the manufacturer’s instructions. pT3-myr-AKT-HA (Addgene plasmid # 31789) and pT3-EF1a-YapS127A (Addgene plasmid # 46049) were gifts from Dr. Xin Chen. .. Lentivirus containing FGFR2-AHCYL1 fusion plasmid and the corresponding vector GV492 were constructed by Genechem Co., Ltd. ICC cell lines, RBE, HuCCT1, and HCCC-9810 were transfected with the FGFR2AHCYL1 lentivirus (multiplicity of infection (MOI)) according to the manufacturer’s instructions.

    Article Title: Multimodule characterization of immune subgroups in intrahepatic cholangiocarcinoma reveals distinct therapeutic vulnerabilities
    Article Snippet: .. We prepared 20 μg of pT3-EF1α-HA-myr-Akt (Addgene, 31789), 30 μg of pT3-EF1a-YAPS127A (Addgene, 86497), 10 μg of pT3-EF1a-KRASWT or 10 μg of pT3-EF1a-KRASG12D, and a 10:1 ratio of transposon to SB-luc transposase–encoding plasmid dissolved in 2 mL of 0.9% NaCl solution. ..

    Article Title: N 7 -Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.
    Article Snippet: .. Briefly, 20 mg pT3-EF1a-HA-myr-AKT (Addgene) and 30 mg pT3-EF1a-YapS127A (Addgene) along with SB transposase plasmid (1/10 25 of the total oncogene plasmid) (Addgene) were diluted in 2.0 mL saline. ..

    Transfection:

    Article Title: A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma
    Article Snippet: The plasmids encoding LDLR were gifts from Marvin Bentley Lab (Addgene plasmid # 162717). .. For transient transfection, plasmids were transiently transfected into ICC cells using Lipofectamine 3000 reagents according to the manufacturer’s instructions. pT3-myr-AKT-HA (Addgene plasmid # 31789) and pT3-EF1a-YapS127A (Addgene plasmid # 46049) were gifts from Dr. Xin Chen. .. Lentivirus containing FGFR2-AHCYL1 fusion plasmid and the corresponding vector GV492 were constructed by Genechem Co., Ltd. ICC cell lines, RBE, HuCCT1, and HCCC-9810 were transfected with the FGFR2-AHCYL1 lentivirus (multiplicity of infection (MOI)) according to the manufacturer’s instructions.

    Article Title: A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma.
    Article Snippet: The plasmids encoding LDLR were gifts fromMarvin Bentley Lab (Addgene plasmid # 162717). .. For transient transfection, plasmids were transiently transfected into ICC cells using Lipofectamine 3000 reagents according to the manufacturer’s instructions. pT3-myr-AKT-HA (Addgene plasmid # 31789) and pT3-EF1a-YapS127A (Addgene plasmid # 46049) were gifts from Dr. Xin Chen. .. Lentivirus containing FGFR2-AHCYL1 fusion plasmid and the corresponding vector GV492 were constructed by Genechem Co., Ltd. ICC cell lines, RBE, HuCCT1, and HCCC-9810 were transfected with the FGFR2AHCYL1 lentivirus (multiplicity of infection (MOI)) according to the manufacturer’s instructions.

    Immunocytochemistry:

    Article Title: A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma
    Article Snippet: The plasmids encoding LDLR were gifts from Marvin Bentley Lab (Addgene plasmid # 162717). .. For transient transfection, plasmids were transiently transfected into ICC cells using Lipofectamine 3000 reagents according to the manufacturer’s instructions. pT3-myr-AKT-HA (Addgene plasmid # 31789) and pT3-EF1a-YapS127A (Addgene plasmid # 46049) were gifts from Dr. Xin Chen. .. Lentivirus containing FGFR2-AHCYL1 fusion plasmid and the corresponding vector GV492 were constructed by Genechem Co., Ltd. ICC cell lines, RBE, HuCCT1, and HCCC-9810 were transfected with the FGFR2-AHCYL1 lentivirus (multiplicity of infection (MOI)) according to the manufacturer’s instructions.

    Article Title: A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma.
    Article Snippet: The plasmids encoding LDLR were gifts fromMarvin Bentley Lab (Addgene plasmid # 162717). .. For transient transfection, plasmids were transiently transfected into ICC cells using Lipofectamine 3000 reagents according to the manufacturer’s instructions. pT3-myr-AKT-HA (Addgene plasmid # 31789) and pT3-EF1a-YapS127A (Addgene plasmid # 46049) were gifts from Dr. Xin Chen. .. Lentivirus containing FGFR2-AHCYL1 fusion plasmid and the corresponding vector GV492 were constructed by Genechem Co., Ltd. ICC cell lines, RBE, HuCCT1, and HCCC-9810 were transfected with the FGFR2AHCYL1 lentivirus (multiplicity of infection (MOI)) according to the manufacturer’s instructions.

    Saline:

    Article Title: N 7 -Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression.
    Article Snippet: .. Briefly, 20 mg pT3-EF1a-HA-myr-AKT (Addgene) and 30 mg pT3-EF1a-YapS127A (Addgene) along with SB transposase plasmid (1/10 25 of the total oncogene plasmid) (Addgene) were diluted in 2.0 mL saline. ..



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